Тақсир, методика йўқ эканми? Ёки, энг содда усули, "до" и "после" посев қилиб, ҳужайра титрини санаш керак керак. Сизда тест-культура борми? (и.колай) 

Dear Bakhtiyor Rasulov,

Thank you for your suggestion, we want to check in vivo antibacterial activity of the substance. In vitro assays already showed that the substance has an activity against E.coli by Disco-diffusion method.

I think in vivo is a complicated task. How do you evaluate the test bacterium in a particular organ? If you spread plate overall dilution from the infected organ or tissue, other cultures also develope colonies along with the test culture. Even in the advanced labs it is not easy to esmitate in vivo. SEM can be useful tool for that, but even in that case you should visually differ the cells of the test pathogen. We have done this, but results were not so reliable. Besides we tested "kill-curve experiments" with E.coli, the method documented in our papers, which you can find here, in researchgate

Thank you for your message but we want to conduct IN VIVO experiment. Therefore we are looking here an advice of experienced people.

OK, sodda metodni topsangiz bizga ham aytvoring, ilojini qilolmayapmiz hech. 

Albatta mavlono, topgan zamonimiz uni e'lon qilish uchun tezlik ila choparlar yo'llagaymiz