Phosphorylation is a key reversible modification that regulates protein function, subcellular localization, complex formation, degradation of proteins and therefore cell signalling networks. With all of these modification results, it is assumed that up to 30% of all proteins may be phosphorylated, some multiple times.
Compared to expression analysis, phosphoproteomics provides additional information, as it provides clues on what protein or pathway might be activated because a change in phosphorylation status almost always reflects a change in protein activity.
Dear Dr. Oliván, ·
The following (two identical articles) papers describe a technique for assessing protein phosphorylation using nonspecific phosphatases that dephosphorylate many phosphoproteins in vitro.
Shenolikar S. Detection of phosphorylation by enzymatic techniques.
Curr Protoc Protein Sci. 2001 May;Chapter 13:Unit13.5. doi:
10.1002/0471140864.ps1305s05. PubMed PMID: 18429117.
Shenolikar S. Detection of phosphorylation by enzymatic techniques.
Curr Protoc Protein Sci. 2001 May;Chapter 13:Unit13.5. doi:
10.1002/0471140864.ps1305s05. PubMed PMID: 18429117.
Best of luck!
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