How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

How to induce doxorubicin resistance in MCF-7 and MDA-MB-231?

I want to establish DOX/R cell line in vitro for the research study.If any one suggest the protocol then it would be a great help.

03 December 2020 9,795 5 View

I teach a STEM career course for IT majors at a small liberal arts school. I am looking for collaborators/information sources for a new book ?

Looking for collaborators/info sources for a new book I am writing in the career/life coaching field on "Adult Bullies". Most research has been done on childhood bullying, however they eventually...

27 November 2020 6,532 1 View

What solvent can I use to prepare a higher concentration of 5-Bromouracil?

I am standardizing a UHPLC method for separation of thioguanine phosphate metabolites. For this, I want to use 5-Bromouracil (5-BU) as an internal standard, however I am unable to prepare solution...

15 November 2020 4,982 3 View

Is annealing should be done again after a year of nano-particles fabrication and they are found to be agglomerated?

I have prepared CeO2 based nano-particles. due to some technical issues some of the properties like electronic structure and magnetic properties are pending. the samples were prepared...

08 October 2020 769 3 View

Advice writing an unbiased qualitative research question?

Hi, I was wondering whether kindly souls out there might be able to help me with some advice regarding the formulation of a RQ for my qualitative study please? I'm trying to refine an early...

25 March 2020 6,512 5 View

Is there any python code which converts text(unstandarized) into a csv fom?

I have an unstandarized text data . So when I convert the text into csv form it results are not into a proper csv format.

05 February 2020 4,556 3 View

Do you think the word/phrase "Economic Growth" is an innocent and positive one?

15 January 2020 2,890 14 View

What ML/AI algorithms can I use to analyze Bank statement and detect frauds??

Ml algorithms which can be useful to analyze bank statement and then extract information about that frauds.

08 January 2020 9,890 6 View

Which book/books are recommended for understanding of diagnosis and management of non healing ulcer? (diabetic ulcers, Venous and arterial ulcers)?

This question is aimed to refer good quality and authentic reference book/text book publication for proper understanding of diagnosis and management of the various non healing ulcers including...

03 September 2019 5,917 7 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Which is the most efficient etching solution for nickel based alloys?

Which solution reveals the microstructure and features of nickel-based alloys?

02 March 2021 2,221 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View