Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View

Tricks to mount clay slides for XRD ?

Does anybody could share some tricks on how to make clay slides (oriented mounts) for XRD diffraction, without having them cracking and/or curling during drying? Thanks

03 July 2020 9,231 4 View

Hi. I am doing a western blotting investigation, and found in different cell lines bands with different weights... Why this hapenned?

I am using anti-fibronectin antibodies in western blotting experiments, and in one breast cancer cell line, I found ~230kDa bands, but in other cell line of this cancer, I found bands of ~35kDa...

02 July 2020 10,015 3 View

In an epidemiological emergency, such as Covid-19 pandemic, are still valid the principles of evidence-based medicine in medical decision making?

EBM suggests that doctors should avoid prescribing treatments that do not have sufficient or strong evidence in their favor. Decisions made backed only by expert opinions and supported by...

02 June 2020 3,700 2 View

How to generate a homodimer from a monomer in PyMol or other software?

I performed tertiary structure prediction of my enzyme in Modeller. However, it is predicted to be a homodimer. How can I obtain a homodimer from the monomer obtained by prediction in a reliable way?

07 April 2020 5,264 2 View

To address the current knowledge on solitary bees (group of species). Which is the best to analyze data ?

Hey everyone, in my thesis one of my goals is to address the current knowledge on solitary bees (group of species). So i made a multiple choice survey covering the basic information about this...

20 February 2018 6,011 2 View

Determination of Kcb and Ke using remote sensing

I would like to know if exist methodologies to calculate the Kcb (basal crop coefficient) and the Ke (soil evaporation coefficient) using remote sensing data (e.g. NDVI, SAVI, etc.)? Thanks,

17 April 2017 4,713 4 View

An Master's student accidentally contaminated the tubes containing restriction enzymes with TAE, so how to removes the TAE from them?

We just bought the enzymes to investigate the polymorphisms in H pylori biopsies. When the student was preparing the G buffer to add in the restriction enzyme tube she added TAE instead ultra pure...

04 September 2016 4,945 3 View

Has anyone worked with a Sigma-Aldrich CHO cell line?

We recently acquired a CHO line from Sigma (00102307) adapted for growth in suspension in Sigma CHO Protein Free Medium. The cells are pink and not growing. Has anyone experienced the same?

19 July 2012 1,730 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View