We are doing many inter varietal hybridization between tropical type (Kenshin) and subtropical type (Chiffu) chinese caggage for germplasm enrichment and selecting high yielding desired genotypes. For shortening the breeding cycle and selecting stable lines, we are planning to do microspore culture using F1 as donor parent. However, in case of this types of crosses we failed to get any microspore derived embryo, whereas this same protocol give very good results in case of the donor of the Kenshin type but never the Chiffu type.
The regenerative ability in microspore culture of Chinese cabbage is under genetic control. My former host researcher in Japan, Y Kuginuki published some artices on this matter. There are much more Chinese reports.
Kuginuki, Y., Nakamura, K., Hida, K.I. and Yosikawa, H., 1997. Varietal differences in embryogenic and regenerative ability in microspore culture of Chinese cabbage (Brassica rapa L. ssp. pekinensis). Japanese Journal of Breeding, 47(4), pp.341-346.
Ajisaka, H., Kuginuki, Y., Shiratori, M., Ishiguro, K., Enomoto, S. and Hirai, M., 1999. Mapping of loci affecting the cultural efficiency of microspore culture of Brassica rapa L. syn. campestris L. using DNA polymorphism. Breeding science, 49(3), pp.187-192.