Question about atomic charges on bio-molecule?

How to assign atomic charges on bio-molecules which were built by a Glycam Carbohydrate builder? The (.pdb) files generated by carbo-builder are without charges of the atom,? Any help would be...

03 December 2020 8,964 1 View

How to determine liquid film thickness (experimentally and theoretically), if the film is floating on another immiscible liquid?

I would like to determine the thickness of a liquid film floating (uniformly/non-uniformly) on another immiscible liquid. How to photograph the cross section of the film (in a non-invasive manner)...

03 November 2020 5,514 3 View

Is antigen retrieval step required for frozen spinal cord sections in IHC ?

I am doing co-immunostain using frozen spinal cord sections for IHC. Is it required to perform the antigen retrieval step prior to the blocking step ?

20 August 2020 4,144 0 View

After resubmitting the corrected version of my manuscript, why does it is showing as "Editor Assigned" for more than 2 months?

Based on learned reviewers given comments and suggestions I have made corrections of my manuscript and resubmit the updated version. In this stage why does it is showing as "Editor Assigned" for...

25 July 2020 5,162 3 View

Steered Molecular Dynamics Simulation using AMBER?

Hi all, I am performing steered molecular dynamics simulation of two polymer chains parallel to each other. Then, one chain has pulled using constant velocity and the trajectory I got is...

23 July 2020 7,805 2 View

Which one is floxed? What are the other methods to confirm it?

I have to collect mice embryos for my research and genotype them for KO gene. In PCR, the floxed band is always blur compare to control (control DNA from adult mice) (please see the attached...

02 June 2020 4,234 3 View

Why does the handling editor asked for major revision?

i. Although the suggested correction was very less raised by the 2 reviewers in their 2nd revision, why does the editor asked for major revision? ii. Why does the editor has given me clues to...

01 June 2020 711 12 View

Is it possible to extract a particular feature from an image based on colour as a shape file using python?

I have a georeferenced image . Is it possible to extract a particular feature like drainage or roads from the geotiff based on its colour and save it as a shapefile using python?

16 March 2020 6,582 1 View

How to identify similar patterns in drainage shapefile using python?

I have a drainage/ river shapefile. If i identify a particular pattern/ feature in the drainage file then using it as input is it possible to identify similar features in the rest of the drainage...

29 January 2020 9,526 3 View

Which is the best model?

Hi everybody, I'm using brms package in R for logistic regression. I compared two models using the 'looic' command. The output contains the following information: Output of model...

04 January 2020 1,074 1 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View

How to identify what type of fillers are in a polymer?

Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...

24 February 2021 1,356 8 View