I am growing Morris cells in petri dishes, and unlike culture in flask, most cells remain suspended. I am wondering if it could be normal or may there be any specific reasons?
Phase quantification in steel contain BCC and FCC
20 July 2024 8,150 7 View
What's your suggestion for converting a heavy organic alcohol to its corresponding acid (via oxidation or dehydrogenation)? Your industrial experience is appreciated.
12 June 2024 3,862 3 View
Need the questions for a quantitative research.
07 June 2024 4,229 1 View
I am analyzing my data using Permutation Tests. I have the codes for a permutation test for the t-test, but I need codes for a permutation test for repeated measure ANOVA and a permutation test...
01 June 2024 1,211 2 View
Hello all, I am doing some heavy simulations in Fluent using clusters. The models are not readable by laptop or desktop because they are very heavy. So, i have to take the contours in cluster...
25 May 2024 6,099 3 View
Hello everyone, I have a dataset of videos for action classification, where each video contains multiple actions. I need to annotate these videos with the name of each class and the start and end...
17 May 2024 5,293 2 View
I need to know the speciation of Cerium in the presence of major anions in several pH, especially Cerium Nitrate. I couldn't find any paper about it. I would appreciate it if someone could...
12 May 2024 1,971 2 View
Which patching method is suitable and practical for training and testing deep learning networks when it comes to patching input data? Is it small patches with overlap, such as 50x50 on individual...
04 May 2024 6,887 3 View
Hi. In my XPS spectra of Co, doesn't exist 2p1/2 peak. But I can see 2p3/2 peak. Is this possible? If possible, what is the reason? Thank you
30 March 2024 8,652 2 View
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26 January 2024 5,164 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View