Hi !

We have performed transformation of STBL2 E. Coli with a PCMV - SCN1A expression vector. We have succeeded in these transformations assays (PCR and Sequencing checked) and we stocked transformed cells in glycerol -80C. After 6-12 months, we grown successfully these glycerol -80C stocked cells in LB solid medium with Kanamycin. We picked positive colonies and grown successfully in liquid LB with kanamicyn. However, our midiprep/miniprep yeld was very low (> 0.1ng/ul). We evaluated kanamycin and midiprep/miniprep reagents with other E. Coli lineages and we could show that all reagents that we used to perform selection and plasmid isolation are OK. So...I would like to ask you if a PCMV vector could integrate into STBL2 E. Coli genome ?