Fabio R Torres

4 Questions 4 Answers 0 Followers

Questions related from Fabio R Torres

How to eliminate late qPCR cycles when N2 target gene is tested for SARSCOV2?

Hi Our PCR plates are showing a high proportion of late amplification for N2 gene (CT>34). In order to minimize false negative results, we would like to diminish the late amplification of N2...

08 February 2022 7,399 2 View

Primers and probe to detect and discriminate P.1 (Brazil) Sars-cov-2 variant using RT-PCR?

Hi guys ! I´m working in a lab that performs RT-PCR detection of SARS-COV-2. We would like to discriminate Brazilian variant from other lineages. Could you provide protocol or primer/probe...

17 March 2021 121 2 View

Could a PCMV vector integrate in STBL2 E. Coli genome ?

Hi ! We have performed transformation of STBL2 E. Coli with a PCMV - SCN1A expression vector. We have succeeded in these transformations assays (PCR and Sequencing checked) and we stocked...

02 August 2018 2,739 2 View

Where can i download seed regions hg19 coordinates of all human micrornas ?

Hi ! I'm interested in genomic regions that code for microRNAs, however i would like to have access to the genomic coordinates of all human microRNAs seed regions. Thanks

16 May 2018 8,136 4 View