What is the best book for finding characteristics group frequencies in Raman?

I need to make some assignments in my Raman spectra and want to use a book to guide them.

10 November 2019 2,290 3 View

How to batch import the Raman spectrum of several log files in gaussian09?

I have dozens of log files and I will like to know if there is a way to batch import their Raman spectrum files from their log file. I know how to import the Raman spectrum of each file by using...

07 August 2019 4,357 3 View

Is it possible for a protein to bind to a weak cation exchanger at a pH above its pI?

I'm reading about the schemes used to purify a protein from different sources. In one of these schemes, they used buffers with a pH (6.5) above the pI (5.7) of the protein to equilibrate a weak...

03 April 2019 8,662 4 View

pH-effect on the Raman frequency

It is possible that if a group in a bound substrate is exposed to the solvent surrounding the protein complex while the rest of the substrate is buried inside the protein, the pH of the solvent...

06 July 2017 2,822 3 View

Getting the x-intercept instead of the y-intercept in Excel Regression analysis?

06 July 2016 3,860 6 View

How can I decrease the fluorescence of my samples for Raman spectroscopy?

11 December 2014 387 12 View

Which supplier sells IPTG at a low price?

I need to buy 100 grams of IPTG and the suppliers I have checked charge a small fortune for this amount. Can someone recommend a supplier that sells this reagent at an affordable price?

02 March 2014 7,231 6 View

Does anyone know a US based company that does sub cloning?

I need my gene of interest to be subcloned into a bacterial expression vector (pET) to produce a N-terminal His tagged protein cleavable with TEV protease. Also, I want Ampicillin to be the...

01 February 2014 1,619 4 View

Has anyone used a Cold-Shock Expression System employing the pCold vector in E. coli?

I am thinking in using this expression system to express dihydropteridine reductase. I want to know if anyone has used this system before and what advantages and disadvantages they have over other...

01 February 2014 1,146 4 View

Can anyone recommend me an ion-exchange column and a gel filtration column?

I need to buy an ion-exchange column and a gel filtration column for a Biologic Duoflow chromatography system. Does anyone knows of any vendors besides Bio-rad that sell these columns? Thanks for...

09 October 2013 9,982 20 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to solve error "Cannot find position restraint file restraint.gro (option -r)." on GROMACS?

Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...

01 February 2021 780 3 View

How to change medium of a protein or peptide mixture?

Hi everyone! I am purifying a peptide from a mixture of proteins. The peptide (3 kDA) is in a solution containing a high concentration of urea and imidazole. I would like to be able to change the...

27 January 2021 4,055 4 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

Any stable dye to color acrylamide hydrogel beads?

Hi! We are generating hydrogel beads(50-60um) using microfluidics as single-cell beads. To simply all the experiments (especially the centrifuge), I am looking for a dye. The dye should be stable...

14 January 2021 4,383 8 View