Hi!
Has anyone find any difference about keeping its slides obtained by cryosection in -80 or -20? I have been trying different combinations as freezing them fast in -80 and then store them at -20, froze them at -20 or directly kept them at -80 without finding any differences. Just curious, as normally we all have the available space in our -80's really limited if we can keep them at -20 without any problem.
Thanks,
Acacio F. Nogueira
For short and medium-term storage of fixed tissue, it is ok to keep in -20, but for long-term storage and preservation of fresh tissues, it is recommended to keep in -80.
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