09 March 2018 1 5K Report

Hi,

To concentrate a protein, what speed should be used in centrifugal filter? Does protein stick to membrane? Some people say that concentrating your protein will lead to aggregation, is it true??

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What false acupuncture points should be included for the sham group in the design of a clinical trial investigating the effect of acupressure therapy?

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Expression of gene from TA sytem in E.coli?

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FLAG tag Immunoprecipitation?

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Ribosome Binding Site E.coli pBAD?

Hi, I am traying to optimize the expression level of my protein cloned in pBAD vector. Currently, I am inducing it at 0.002 w/v but see a significant overexpression of my protein. The current...

26 May 2021 6,335 2 View

Duet vectors with Arabinose promoter?

Hi, Does anyone know of any plasmid where I can clone two genes under two promoters? Basically, I want to coexpress these protein together but with Arabinose inducible system. pETDuet,...

07 May 2020 8,328 0 View

Membrane anchored protein?

Hi, Is there any way to prove that a protein is membrane anchored/peripheral membrane protein? Any simple trick or any technique will be helpful. Thanks

24 November 2019 1,152 5 View

Detergent for bacterial cell lysis?

Hi, Can someone please suggest which detergents to use in regular lysis buffer/resuspension buffer for E. coli cells? Please also suggest the optimum concentration to be used. Thanks.

24 November 2019 1,485 5 View

Designing mutation experiments??

Hi all, I have a protein with conserved Threonine and one Lysine in the middle of the protein sequence. Being a charged and conserved amino acid across family domain of this protein, I have strong...

21 March 2019 3,201 4 View

E.Coli expression strain for expressing mycobacterial membrane protein gene?

Hi, I have cloned some three different constructs which are putative membrane protein of Mycobacterium tuberculosis into pET28a with N-term His tag. I tried to express them in BL21, Rosetta, C41,...

17 December 2018 8,644 2 View

Use of Agarose Low EEO??

Hi, Can anyone please highlight the difference between normal agarose and agarose low EEO? Any specification as to which to use for agarose gel electrophoresis? Is it that extraction of pcr band...

30 November 2018 6,802 3 View

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