I expressed my effector protein in Pichia pastoris using BMGY (10 ml media until OD 2-6) AND BMMY (adjusted to OD1.0 and methanol induction 0.5% daily) and harvested the protein. In the supernatant samples I did SDS PAGE and western blot and the bands are very faint - indicating low expeession. there are clear bands hut not of my protein size when I precipitated with acetone. Can I still use it for leave infiltration? Since I wanna check for necrosis/hypersensitive response in Banana leaves?

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