Cindy Octaviana

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Questions related from Cindy Octaviana

Concentrate secreted protein expressed in Pichia pastoris even when there is low expression?

I expressed my effector protein in Pichia pastoris using BMGY (10 ml media until OD 2-6) AND BMMY (adjusted to OD1.0 and methanol induction 0.5% daily) and harvested the protein. In the...

04 May 2025 5,309 2 View

Is it better to do acetone precipitation for my proteins from Pichia expression before western blotting?

I produced some small secreted proteins (fungal effector proteins) with Pichia pastoris system and I have the supernatant samples (in BMMY media) to check for the expression. I am not sure if it...

17 April 2025 2,822 3 View

SDS PAGE blurry bands Pichia pastoris crude supernatant?

I recently expressed my proteins with P. pastoris GS115 and then harvesting 1 mL samples for different induction times to see my effector proteins expression. So the protein samples are frozen at...

09 April 2025 6,464 4 View