I have to purify a glycoprotein from tissue homogenate for which i have to perform affinity column chromatography on Concavalin-A agarose column. How can i prepare a column for that rather than buying it?
You can activate agarose using epichlorohydrin or 1,4 butanediol diglycidyl ether, then you can attach con A.
Hi, you can follow the paper attached bellow.
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or we can collaborate
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