I have to purify a glycoprotein from tissue homogenate for which i have to perform affinity column chromatography on Concavalin-A agarose column. How can i prepare a column for that rather than buying it?
You can activate agarose using epichlorohydrin or 1,4 butanediol diglycidyl ether, then you can attach con A.
Hi, you can follow the paper attached bellow.
Pentachloronitrobenzene is used to prepare the "Nash & Snyder" media to isolate the Fusarium. But this is not dissolving in 75% ethanol and distilled water (Normal and hot both). So Please...
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Please tell me about the rate (in SCCM unit) of the flow of gases and pressure inside the tube.
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Some Mathematical expressions will be helpful.
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Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...
03 March 2021 4,275 4 View
Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
03 March 2021 6,299 3 View
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I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...
02 March 2021 1,408 1 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
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Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
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Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
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In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...
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