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I am trying to purify leptin which is a glycoprotein via affinity chromatography. The molecular weight of my protein is 16 KDa. With the help of certain papers i am going to perform it in...
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I have to purify a glycoprotein from tissue homogenate for which i have to perform affinity column chromatography on Concavalin-A agarose column. How can i prepare a column for that rather than...
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Is Folch lipid extraction method suitable for estimation of total lipids from animal tissue, if not may I know some other suitable and reliable methods?
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Can someone please tell me reliable and simple methods to estimate minute quantities of glycogen, lipids and phophorus in an animal tissue?
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I am following TCA method for sample preparation for 2D- Electrophoresis. But the very first step say prepare a cell lysate in which there should be 2-3mg protein per ml. So my question is how to...
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