Cell Culture Protocol for 67NR and 4T07 cells?

Hello there! My lab has recently been (very graciously) gifted an array of cell lines, of which include 67NR and 4T07 cell lines. While I've found a decent amount of background and culturing...

02 February 2021 2,590 3 View

How can I calculate Standard Deviation using DataWarrior?

I am analyzing about 500 results in DataWarrior and I need to calculate the mean value and the SD. I managed to calculate the mean value using excel, but it I couln't find a way to simply...

25 October 2020 9,318 3 View

Escalating dose regimen with osmotic mini pump?

Hi everyone, I wonder if someone has ever used osmotic mini-pump for an escalating dose regimen. I read that some iPRECIO osmotic mini-pump are programmable and therefore can be used for infusion...

03 April 2020 9,009 3 View

Is there anyone that have a trasfection protocol optimized for suspension cell culture?

We work with WIL2 cell line and want to trasfect mirna mimic and inhibitor. We tried first with lipofectamin and than electroporation (using amaxa nucleofector, U-001 program), none of that works....

27 March 2017 4,176 4 View

IWP-2 inhibitor

16 February 2016 1,730 4 View

Is there any solvent to solve SEBS blend?

I need to know the amount of oil present as plasticizer, so I would like to know il i can dissolve SEBS in one solvent and then recrystallyze it in another solvent.

30 September 2015 9,013 2 View

Could you help me with a Capitellidae identification?

Hi, I'm working with samples of the Portuguese Continental Shelf; I found few specimens of Capitellidae with 11 thoracic segments, in which the first is achaetigerous and the other 10 with only...

19 July 2015 1,087 4 View

Is anyone working with PK15 cell line free form circovirus?

I would like to find PK15 cell line free of Circovirus to attempt PCV2 isolation.

06 May 2015 6,617 2 View

Does any body explain why there is a superheater stage in the organic rankine cycles (ORC)?

I am interested in ORC-system. If I look to the hp-diagrams of the main working fluid they do not need a superheating stage, but in reality I saw that in many ORC the fluid is also superheated....

18 February 2015 10,018 3 View

Is anyone working on seasonal thermal storage based on absorption processes?

In our institute we want to develop a seasonal thermal storage system based on absorption process. I know that a project based on this principle is running at the Empa (Switzerland) but I did not...

18 February 2015 9,094 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

The procedure compute?

02 March 2021 5,204 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

If you haven't computer? how you show the photons to pupils?

Educational Process

01 March 2021 1,827 1 View