What is my next move if almost all Inter-item correlations are above .7?

I am doing a validation study for my master thesis, but am having some difficulties in figuring out my next steps. My reliability is showing a very high chronbachs alpha, but my inter-item...

16 February 2020 9,909 3 View

Assessment of air microflora in the microbiology Laboratory?

To assess the air microflora in indoor air of laboratories

14 January 2020 2,006 4 View

How to collect Oil during pyrolysis in a Tubular Furnace?

Hello I am using a tubular Furnace (1100oC) for pyrolysis at 300oC. After the first pyrolysis, the quartz tube was soiled with bio oil my problem is, the quartz tube is enclosed so how to...

14 January 2020 724 4 View

How to determine pH of Biochar ?

i want to run some adsorption test using biochar but i want to check the pH first must i necessarily follow the IBI Biochar international guideline ? or i can determine using another method? can...

23 October 2019 7,102 6 View

How can I measure the color of water by image processing?

Hi everyone The water color is affected by the color of its surroundings. How can I measure the color of water by image processing? Should white paper be placed under its glass container?

17 December 2018 5,150 3 View

How to run residual stress relaxation by cyclic loading in Abaqus? Which solver to used if load applied is tensile (max 19kN and min 1.9kN)?

Residual stress relaxation due to cyclic loading (tension-tension).

06 June 2018 6,168 3 View

What are the possible reasons why antibodies may fail to neutralize a toxin?Why is binding separate from neutralization? Any explanations?

Monoclonal antibodies were generated against a toxin. But in vitro neutralization assay has shown the antibodies not able to neutralize the toxin.

29 June 2017 3,009 7 View

How to parameterize ligand in MD simulation using VMD-NAMD?

14 September 2016 9,898 4 View

Problem of using command prompt window in molecular dynamic simulation?

I want to perform simulation and when I run the command prompt window with the following syntax " namd2 ubq_ws_eq.conf > ubq_ws_eq.log &" below error will appear" Charm++ fatal error:FATAL...

07 July 2016 8,576 18 View

How do I generate DOPE score using multiple template (model_mult.py) in modeller 9.16?

I want to build a model of query sequence (qseq) based on the alignment against the multiple templates using the `model_mult.py'. from modeller import *from modeller.automodel import * env =...

18 June 2016 6,786 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

How to revive a Hyphomicrobium strain?

11 February 2021 3,524 2 View

Bradford assay for insulin: discrepancies between standard curve and sample concentration during crystallisation?

Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...

06 February 2021 9,370 7 View

How to measure volume of Pinus roxburghii ?

I want to measure volume of Pinus roxburghii in the Jammu region of India to calculate the carbon stocks of Pinus roxburghii.

31 January 2021 2,311 3 View

Where can I find the information about steel final production in Japan?

I am doing research about the in-use stock of iron and steel in Japan. I need the report about annual yield of steel final production. I found some information in the webpage of Japan Iron and...

25 January 2021 3,867 2 View

How to revive bacteria from glycerol stock?

19 January 2021 7,025 4 View

How can I prepare the erythromycin stock?

I need to prepare a stock of 10 mg / ml erythromycin to check the mic value. I am trying to dissolve 0.1g/10 ml of water but i can't dissolve erythromycin in water. How can i prepare 10 mg/ml...

03 January 2021 9,947 3 View

Which form of tryptophan and tyrosine is highly soluble in water?

The solubility of tryptophan and tyrosine in water is low. Is there a very highly soluble structurally similar or derivatives of these amino acids I can use? it doesn't matter if it is...

20 December 2020 3,157 3 View