I'm not sure about one thing.. Hope any analytical biochemist or someone who knows a bit on that side can help. The question is : "In order to compare the protein to lipid ratio of any biological sample, along with the type of lipid content, which way is better: UV/NIR spectroscopy, FTIR, HPLC or do all 3?" PS: LC/MS is not an available option.
Hello Maliha,
For making your work simple, you can measure protein and lipid content separately of the sample. And for lipid type analysis, you can perform gas chromatography to know fatty acids type.
Im interested in membrane proteins and lipids of intact cells and intracellular vesicles..Membrane topology basically. So i cant do it separately. Any other suggestions?
@Katarzyna M Marzec. Madam is Raman used for lipids? Yes it will do well with proteins.
- Badges
- Science topic
- Similar topics
- Papers