Can anyone please recommend a manufacturer of the kits for microRNA hybridization and detection by FlowFISH method except eBioscience?
This year we have the opportunity to purchase an HRMSD. We have an Agilent Infinity II 1260 HPLC in our lab and are planning to purchase a Q-TOF. I have experience with Agilent equipment...
26 February 2024 7,577 6 View
What methods are best used in the statistical analysis of ultrathin cell sections for transmission electron microscopy? I need to estimate the number of vesicles 50-100 nm in size in different...
01 April 2021 3,522 2 View
Dear colleagues, We have Agilent GC/MS 6890N/5973 inert with turbomolecular pump. I would like to use it for analysis of nitrogen fixation activity of bacteria by determination of ethylene and...
17 January 2016 140 16 View
Hi everyone,I need advice about mass-spectra identification collected in LC/MS (single MS mode). Spectra in *.raw and *.cdf.Is it possible to make an identification offline with freeware software...
28 November 2014 9,167 4 View
The desired thickness is ~1.5um. i-line resist.
05 July 2012 3,866 5 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...
28 July 2024 5,658 0 View
Dear colleagues, We are trying to do FISH on mice PDEC metaphase spreads. We use homemade biotin labelled probes, synthetized using a nick translation kit. For hybridization, initially we were...
08 July 2024 8,687 3 View
Hi everyone, I am working on a hybridization chain reaction (HCR) and trying to visualize it with gel electrophoresis. The visualization of the HCR works but for the single components of this...
07 July 2024 9,413 4 View
Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...
24 June 2024 3,507 2 View
Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...
18 June 2024 2,799 4 View
Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...
16 June 2024 9,723 2 View
Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...
06 June 2024 5,715 4 View
Why we checking the GCA of a parent during hybridization program?
26 May 2024 590 1 View