Dear Colleagues,

I wish to perform a genotype-phenotype correlation study in a family with a known mutation in RPGR-ORF15.

However, the RPGR-ORF15 region is very tricky because it contains GC repeats and thus its not possible to amplify with standard PCR conditions.

Special protocols are needed for both PCR amplification as well as during Sanger sequencing.

Therefore, I would greatly appreciate if anyone could suggest me a commercial facility where I can send my samples for screening the specific mutation located in the RPGR-ORF15 region?

Number of samples to be screened = 05

Many thanks,

Atta