Please help me to understand the flow pattern of mobile phase inside the column.
please share any link where i can update myself.
Thank & Regard
With regard, Please suggest that have you experiences straight HPLC column selection by knowing molecular structure. or what are the concept you follows for selection of HPLC column. (i.e....
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when formulating a delayed release dosage form what the differences are undertaken in various delayed release formulation e.g.Binders, compression.
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Is there any difference in principle of reverse phase chromatography and Normal Phase Chromatography? Please share any link where i can update myself in detail. Thanks and Regard
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I'm using Chiralpak IA column for my analysis. initially there was good resolution in my analysis, but followed by time the both peak in racemic merged. My mobile phase is only n-Hexane:IPA...
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I used Chiralpak AD-H column, and my molecule having THF, MDC in traces. So within 1 month the column gives a poor peak and also tailing with peak broadening. I tried ethanol, IPA for washing as...
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A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
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Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
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I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
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I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...
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