Correlation between molecular structure and column selection?

With regard, Please suggest that have you experiences straight HPLC column selection by knowing molecular structure. or what are the concept you follows for selection of HPLC column. (i.e....

08 September 2019 846 4 View

Correlation coefficient limit in HPLC method validation

05 June 2016 4,040 1 View

WHAT ARE THE MANUFACTURING DIFFERENCE TO FORMULATE A CONTROLLED RELEASE AND SUSTAINED RELEASE DOSAGE FORM?

when formulating a delayed release dosage form what the differences are undertaken in various delayed release formulation e.g.Binders, compression.

10 November 2014 3,890 0 View

How can you know that a sample is uv active?

Please update me- is there any specification on structure basis, that a compound is UV active? As a large molecule, can we make a decision to look into whether the structure of that molecule is UV...

10 November 2014 2,276 7 View

What is the entropy of a dead man?

i had the query to understanding about thermodynamics law. recently i found that thermodynamics is unable to provide a reason behind the entropy of living organism. so how can we know that what is...

10 November 2014 1,835 1 View

Can anyone explain the different principles of HPLC?

Is there any difference in principle of reverse phase chromatography and Normal Phase Chromatography? Please share any link where i can update myself in detail. Thanks and Regard

08 September 2014 5,360 21 View

How can I improve my chiral column resolution?

I'm using Chiralpak IA column for my analysis. initially there was good resolution in my analysis, but followed by time the both peak in racemic merged. My mobile phase is only n-Hexane:IPA...

08 September 2014 4,681 9 View

Problem With CHIRAL PAK AD-H Column - Can anyone help?

I used Chiralpak AD-H column, and my molecule having THF, MDC in traces. So within 1 month the column gives a poor peak and also tailing with peak broadening. I tried ethanol, IPA for washing as...

08 September 2014 7,455 13 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View