Hi,
I would like to know would it be possible to measure the colocalisation between 2 dyes / stains in the IHC stained tissue using ImageJ colocalisation algorithm. I notice that the image J colocaliation algorithm only used for fluorescence stained tissue. We have another option to use colocalisation algorithm by aperio, but it costs a lot.
KIndly advise me for any software that able to measure dual color / colocalisation in IHC stained tissues.
I truely appreciate all the replies and suggestions.
Thank you.
If I understand correctly, you are trying to analyse co-localisation in an enzymatically stained sample? So the problem is that you can't distinguish the 2 separate stainings in separate channels? In that case I believe you can try to use an ImageJ colour deconvolution plugin, such as is available in FIJI. After that, if the deconvolution was successful (it might need optimisation), you might be able to do colocalisation analyses. I hope that helps.
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