I´m trying to co-culture nonadhesive cells (Jurkat) with adhesive cells (macrophages), using Transwell dishes from Corning. Could someone help me with the protocol???
How long do you have to cultivate them? How big are the pores? This 2 conditions can make a difference in the growth of the cells. If the pores are to big, the macrophages will tend to go thorugh if the incubation time is too long, so you will have the macrophages on the other side of the transwell. I thinks there is no real protocol for such a culture.
You could try to activate the jurkats so they turn adherent, but remember: both cells are VERY motile, so they will migrate through the pores.
Sorry I can not help you more, but I need to know what exactly your hypothesis is, in order to consider all options...
Thanks for your response. My intention is to incubate Jurkat with macrophages, stimulating them and study levels of cytokines produced by Jurkat for further pharmacological studies. The culture time must be at least 72 hours. Any help is welcome
Hi Pilar, well, if you only want to see cytokine production, you can take transwells with less than 1µm pores. There should not be any problem with the growth of both cell types (grow them on the top of the transwell). if you need any more details send me an e-mail through research gate.