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Sequencing of a plsmid?

I did whole plasmid sequencing of a plasmid I received from my colleagues when the result came out why was everything reversed in it? Meaning the format should be 5'promoter-gene-terminator-...

19 June 2024 5,422 3 View

Differential gene expression?

Hello, I am working on the mouse lens, in which I have obtained lens fiber and epithelium at two-time points, E17 (embryonic day 17) and P2 (postnatal day 2) for two different mouse strains,...

29 May 2024 3,367 0 View

Can I get some resource or something so that we can get a idea how to work with sentaurus TCAD workbench?

Resource for working with TCAD workbemch

21 May 2024 2,635 1 View

Has anyone used starbase or lncBase to predict the interactions between miRNA and lncRNA?

Softwares used to determine the interactions or binding sites on lncRNA

28 April 2024 2,745 0 View

What is the Impact of empowering an inquiring mind in a classroom?

History of what lead to this thinking Current issues and debates around this paradigm

01 April 2024 459 0 View

Can anyone help me in functional annotation of lncRNAs? Or if someone has worked with NONCODE please help?

NONCODE is the database used for the different kind of information related to the lncRNAs like disease involvement, orthologs etc.

09 March 2024 7,571 0 View

Cloning of poly A tail?

Hi, I want to add Poly A Tail at the end of my construct after 3'UTR so that I do not have to do Poly A Tailing every time I am making RNA and doing injections in Parhyale. Can someone guide me on...

18 October 2023 5,075 0 View

I am having problem with the cis calculation in gamess with ACCT basis set. I have provided my input and error below. What is the solution?

$CONTRL SCFTYP=RHF CITYP=CIS RUNTYP=ENERGY ISPHER=1 ICHARG=0 MULT=1 $END $CONTRL QMTTOL=1.0E-6 ICUT=11 UNITS=ANGS $END $SCF FDIFF=.T. $END $SYSTEM MWORDS=1000 MEMDDI=1000 $END $BASIS...

11 October 2023 3,701 0 View

Adding Poly A tail?

I want to add poly A tail at the end of 3’UTR so that I don’t have to do poly-A tailing every time I make RNA. Please let me know how I can do it. It should be poly A 200nt correct? should I make...

10 October 2023 351 1 View

Anyone working on lncRNAs? If anyone knows about the screening of the lncRNAs using bioinformatics if pathway is already known?

I already know the pathway but want to know the upstream lncRNAs that regulates that pathway using the datasets and bioinformatics.

08 October 2023 2,798 3 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...

10 August 2024 4,005 2 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

Hakan Lane

Dear Sanjana Goyal Delighted to join, thanks

Sumathi Subbarayan

Hi, I would help you with data analysis.

Sanjana Goyal

Sumathi Subbarayan sure! Does your educational background lie in the field of psychology or nutrition?

@Sanjana Goyal Statistics and Mathematics with Data analysis

Sumathi Subbarayan currently, we are only looking for people with background either in psychology or nutrition. We won't be able to move forward with you, but hope to work with you in future.