E.coli toxic genes.
Thanks for your reply.
I use para nitrophenyl palmitate substrate with tris buffer ph 8... i read many temperature for Yellow color of para nitro phenol production.. 37 and 50 and 40 at water bath ... I confused as i...
04 February 2021 9,100 5 View
If I want to know the changes (for example in plants or soils) as affected by specific substance on nano substance; when I use SEM or TEM and why? As well, when I use FTIR or EDX and why also? Is...
28 January 2021 5,070 9 View
iam working on long memory time series data and i use lstm neural networ to train data and i want to understand how rmse is calculated is it calculated in each iteration separately or in each...
23 December 2020 6,740 4 View
if the percentage of doping Au into rGO is too small 1~10%, is it not enough to prevent restacking of r-GO in xrd results, rGO peak appeared at 22.2 and also the peaks of gold nanoparticles so...
21 December 2020 6,091 3 View
is the concentration of gold salt that were added will affect on the peaks appeared. i added a very little amount of gold salt to dope graphene oxide and not all the peaks that proved the...
17 December 2020 9,275 3 View
i am trying to dope boron in graphene oxide , and after i take out my samples from the oven i put them in the microwave for 40 sec , and i saw sparkles . and fume also i couldn't understand why
16 November 2020 6,852 5 View
i am looking for efficient texture analysis software for MRI images analysis with free access?
09 October 2020 3,737 2 View
Can you please suggest some websites where the MRI images can be downloaded for free.
05 October 2020 2,014 3 View
I would like to analyse fish gut bacteria by the Functional analysis by Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). How is this analysis done?
04 September 2020 3,048 1 View
I have Pre test as a (covariate variable) and post test as a (dependent variable) and the treatment for the experimental group and the control group. and the assumption of Homogeneity of...
21 August 2020 7,196 3 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...
26 February 2021 4,777 4 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...
25 February 2021 5,712 3 View
