I have TEM images of axons, but as expected, they come in a variety of shapes and sizes. They are not all perfect circles, so the classic theory of G-ratio seems to have biases depending on where you measure from.
I have heard people measure the circumference and then work backwards to get g-ratio (doesn't seem fair either to me)
Or also use the area measurements. This I have mostly gotten from Article In vivo histology of the myelin g-ratio with magnetic resona...
But what do axon biologists do. All I read in methods sections of otherwise great papers is "we used Image J" or something similar.
If I can be pointed towards a good protocol or paper that calculates g-ratios in a descriptive and fair manner, I will be very thankful.
I recommend our paper...it's the best! This was the first time my lab measured axons using TEM and I was frustrated by the lack of clear published methods. So when I wrote this I included exact details of our measurement and statistical procedures for axon area, myelin thickness, and g-ratio:
Article Peripheral Axons of the Adult Zebrafish Maxillary Barbel Ext...
Hi. I am not sure if you have reached a conclusion with what method to use, but I recommend this paper. I am currently in my last year of PhD and work on remyelination, and like you I have asked myself the same questions and decided to follow the methods as in this paper. I hope this helps. Best of luck. Article Activin receptors regulate the oligodendrocyte lineage in he...
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