Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Plant research: Watering uniformity/consistency. How important is it?

Trying to gather insight from an unresolved discussion with a colleague here, and either the available literature surrounding this topic and/or my keywords in search of are lacking. Context:...

21 February 2021 6,389 3 View

How to get rough estimation of the concentrations of Oxygen and moisture inside the glove box?

Dear colleagues, I was just wondering if you can suggest a good method to get a rough estimation to the concentration of O2 and H2O inside the glove box as unfortunately, our glove box doesn't...

17 February 2021 1,012 3 View

Onset of Photo Oxidation in Polymers?

Hi! I am looking for some specific information in regards to the minimum amount of UV intensity required to begin the photo degradation process in polymers. I understand each polymer is...

15 February 2021 7,703 4 View

Are you looking for a quality online conference on teaching and learning technology?

This large international conference will be held online this year due to COVID. I am submitting a paper to the Technology Enhanced Language Learning track (8). It is about a topic I have never...

15 February 2021 1,975 15 View

How do you calculate a discrimination index for polytomous data?

Hello, I'm currently running a PCA on a polytomous data set (5 point Likert). I want to create a discrimination index to narrow down my items before running the PCA. After looking around, I'm...

13 February 2021 3,517 2 View

Regarding immunology, transmissivity, herd immunity and vaccines in Sars CoV-2?

This is a follow-up on an earlier question regarding mechanistics of vaccines: why current vaccines don't block transmission. In summary, they don't engage mucosal immune cells to produce IgA....

08 February 2021 8,321 3 View

Which tropical country is the most easy for obtaining research permit (for biological research)?

Dear all, High beurocracy hihly complicate field ecolocial and evolutionary research nowadays. Nearly all tropical countries (maybe all?) requires research permit for performitng research and in...

04 February 2021 3,672 6 View

Can I polymerize NIPPAm with PEGmethacrylate or only with PEGacrylate?

I want to perform the synthesis of a hydrogel and I want to know if I can polymerize NIPPAm with PEGmethacrylate or only with PEGacrylate?

02 February 2021 2,106 6 View

What is the maximum storage time for samples used in soil enzyme assays?

01 February 2021 4,616 3 View

How to assure whether a regression tree overfit or not by seeing bias-variance value of the model?

Lets consider a regression tree in which variance is 1.1065*e-10 and bias is 2.962e-13. Also the model RMSE on training set is 1.5e-5 and on training set is 1.2950e-5. Is the model is overfit or...

22 February 2021 7,755 2 View

Interpretation of Goodman - Kruskal Gamma squared?

Dear colleages, Eta squared is dominantly used in reflecting the explaining power the same manner as a squared partial correlation coefficient (R2p ) from multiple linear regression: as the...

22 February 2021 7,801 3 View

How is the variance for an image exactly computed to test the uniformness of its histogram?

I am trying to compute the variance of a plain and cipher grayscale LENA image... I found in a paper that variance value is equal to 630780 for a plain Lena image while if I calculate I find it...

28 January 2021 8,036 5 View

Reliability of the model created by RSM (Response Surface Methodology)?

When you create a model with RSM, even if the R2 value is large and the result of the analysis of variance is significant, if you look at the individual explanatory variables, if there are many...

22 January 2021 454 1 View

How To Check Time-Series Stationarity in R ?

When validating a time series, one of the first things to check before building an ARIMA model is to verify that the series is stationary. That is, it must be determined that the time series is...

18 January 2021 700 11 View

Does anyone know how to fix the "A sample of parameter values was inadmissible"-error in SPSS AMOS when imputing factors?

Hi all, I'm trying to impute factors after testing my model for CMV and therefore included a common latent factor. Unfortunately, I always get the same error: "A sample of parameter values was...

02 January 2021 6,647 5 View

MANOVA with 1 independent (predictor) variable and multiple DVs: how do I interpret and report the results?

The aim of my thesis is to see whether anxiety score can predict sleep quality and dream content. So for this, there is one continuous variable that I want to use as a predictor on multiple...

27 December 2020 790 8 View

What Explained Variance is good to proceed with Predictive Soil Mapping?

I have 1851 soil samples data on pH covering a study area of 7482sq.km in northern Ghana and I am using 52 environmental long-term average variables (Relief, Climate, MODIS Reflectances and...

27 December 2020 2,728 6 View

Morphogeometrics analyses: how do I know which landmark has the greatest variance?

I'm using MorphoJ, and the software gives me the eigen values but it doesn't give you the eigen vector. For each landmark it gives you a x and y value for each PC but I'm looking for a value that...

21 December 2020 3,466 3 View

How to calculate broad sense heritability from analysis of variance table below the data set below ?What can be the heritability?

Analysis of Variance Table Response :Se content Df Sum Sq Mean Sq F value Pr(>F) environment...

20 December 2020 2,406 1 View