How i can stabilise the chlorophyll pigment (methode of stabilisation)?
Because the chlorophyll is decompose if its exposed under light
I am not sure what kinds of experiments shall be done, however the stability of Chlorophyll will rise if working under oxygen free atmosphere. As this might be complicated an assay for depleting oxygen in solution might help. We used a mixture of glucose, glucoseoxidase and katalase to speed up oxidation of glucose ensuring an oxygen free atmosphere. You can check this by lifetime of the Chl triplets which are not longer able to react with oxygen triplets in the surrounding.
Details are described here:
Article An unusual role for the phytyl chains in the photoprotection...
Thank you yes if the chlorophyll its excited the electron of chlorophyll is transfered to oxygene and form high reactive radical oxygen they decompose the chlorophyll. The remplacement of mg by cu its more stable?
And if i add material have good oxygene barrier?
If a replacement of Mg is possible that would be an option. Another possibility is to use Chl embedded in protein which forms an excellent oxygen barrier or- as recently shown, prevents formation of singlet oxygen. We hav used WSCP which is a quite cheap protein embedding 4 Chl and the Chl inside is pretty stable for weeks under radiation:
Article Water soluble chlorophyll binding protein of higher plants: ...
Okk thank you but i want srability for not one week only ( increase the time of stability for a 3 mont for exemple)
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