For your case, if you have access to DCCC (Droplet Counter Current Chromatography) will be more feasible. Size exclusion will also work but you have to choose the right pore size and MW specification Sepahdex/Sephacryl/Sepharose. As for the Cellulose is concerned, you may need to swell it for 24-48 hours (don't boil instead keep it at room temp soaked or very gentle stirring) in a solvent (you need to make the slurry, the solvent should be very pure, preferably double distilled, ions free, metals free, degradants free) before you load and start eluting your mixture's compounds. A small-scale pilot trial is a must to familiarize and learn the nuisances.

Riaz A. Khan thank you so much for your input. We have a lab equipped with the instruments for analytical testing. However, for this particular project we are trying synthesis and separation from natural resources. We are trying conventional chemistry however it did not worked.

As suggested by yourself I will try soaking cellulose in butanol saturated with water hoping for the best and will share my experience. In the meantime further suggestions will be appreciated.

Riaz A. Khan I tired different conditions of using Cellulose (soaked in water, and in n-butanol saturated with water for 24 to 72 hrs.) but the maximum elution rate which I got is about 30mL per hour under the impact of nitrogen gas pressure.

Any suggestions to improve the elution rate using the same solvent? Thanks

Try VLC (Vacuum Liquid Chromatography), expected to be better than the Flas/Nitrogen Pressure Chromatography.