I wonder how to effectively stain cellulose nanofibers for improved resolution in TEM (120 kV). Recipes would be highly appreciated and possibly micrographs. Anyone compared different methods?
All the best and thanks for input.
Conventional negative staining with uranyl acetate works well.
Good luck!
There is a protocol:
https://www.researchgate.net/post/Does_anybody_have_a_protocol_for_uranyl_acetate_staining_of_nanocellulose
120 kV does not have sense. 80 kV (conventionally used for NS samples) is enough. The problem is not in "optical" resolution. Support film thickness and staining are more important. I strictly recommend to use the ultrathin formvar films:
Article Ultrathin Formvar Support Films for Transmission Electron Microscopy
Good luck!
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