27 August 2020 1 4K Report
  • I want to conduct some study on PCOS patient's endometrial receptivity. I want to build a cell model that simulates the endometrium of a PCOS patient. But my experience in this matter is too limited. Could you help me to figure out, please?

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Quantification of CD8 positive cells using ImageJ software?

Hello everyone, I am working with mammary fat tumors from mice. My goal is to characterize tumor-infiltrating immune cells between different tx groups. I did immunofluorescence staining with...

01 July 2024 8,964 1 View

Can we use different Likert point scale for SEM-PLS ?

We have two sets of data, and we want to conduct SEM-PLS and multi-group analysis via SmartPls. However, one set of data is 5 point Likert scale while another is 7 point Likert scale. Do I need to...

21 May 2024 8,548 4 View

Quadratic Mixed Model Assumptions?

Hello, I am new to mixed effects model and am running a analysis with it. However, am wondering if the assumptions check for Quadratic mixed models are the same as for linear mixed model? -...

15 May 2024 7,716 3 View

Help me to find XRD jcpds card information?

I would be extremely grateful if someone could help me find the following cards: CaZnOS PDF-01-072-3547 SrZnOS PDF card # 041-0551 BaZnOS PDF #17-1239 Ba0.5Na0.5TiO3 - SrTiO3 Thanks a lot!

08 May 2024 5,034 1 View

How to protect human DNA during centrifugation?

I have been trying to collect bacteria from human fecal sample to run on MT-PCR, however, for some reason, the concentration of human DNA is quite low and could not confirmed on PCR. My technique...

07 May 2024 8,173 1 View

What is the meaning of depth and level in Imaris statistic analysis (filament function)?

I used filament tracing of Imaris to reconstruct dendritic spine and I got the statistic result of dendritic spine density. I wanted to count dendritic spine density in secondary and tertiary...

06 May 2024 8,675 0 View

Why protein bands are unclear or invisible on SDS PAGE gel after staining?

Hi everyone! I have carried out SDS PAGE analysis many times. In the experiment, protein sample was hydrolyzed gelatin. After staining, only bands of protein marker (standard) were shown, whereas...

24 April 2024 6,444 1 View

Hello! I hope you're well. How can I establish the molecular equation of a biomass sample? Any recommended techniques?

I utilize a photosynthesis reactor for wastewater treatment. Within the reactor, I collect biomass and aim to establish its molecular equation of biomass, such as CxHyNzPtKi.... I would greatly...

20 April 2024 6,384 6 View

How to Handle Multiple RTF and PRM Files with the charmm2lammps.pl Script?

I have previously used the charmm2lammps tool to generate LAMMPS inputs multiple times, specifically for simple protein systems. For those systems, I was able to create LAMMPS input from a PSF+PDB...

14 April 2024 8,470 0 View

How do I determine sample size with G*Power for moderation analysis (Hayes Model 1)?

Hello I am trying to run a moderation analysis but will need to use G*Power to determine my sample size. Just wondering if anyone could assist me with the following: 1) the parameters to set 2)...

08 April 2024 7,405 7 View

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Could it be a cell culture contamination?

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

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07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

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Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

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Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

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"A Markov-like Model for Patient Progression"?

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How to report results of Generalised Linear Mixed Models in a journal article?

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How to isolate lymphocytes from mouse spleen?

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How to Add Missing Water Molecules in Protein-Membrane Simulations?

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