A new student in my lab was passing my cells the other day and didn't check the temp of the water bath. Someone had turned up the temp thus PBS and media got heated to 56 degrees C. Most of the cells died. I'm wondering if I should toss the media?
Hi. I have the same problem. ¿What was your outcome?
The cells died!
I mean, with the media. ¿Did you toss it?
Oops! Yes, we didn't want to risk using it on any cells after that.
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