Hi,

I would suggest that instead of going with Manual counting,You can directly use kit for proliferation studies either MTT or XTT, this will give you an absorbance at specific wavelength and you can plot the growth curve based on days Vs Absorbance..

Thank you.

Jyotsna

Hi,

you can try to transfer cell suspension in a eppendorf while you count another well. 

However, I think that a cell detached by trypsin isn't able to attach in a so little gap of time. Which method do you use to count cells? Burker chamber or cell counter instrument?

At the same time, I think that using MTT or XTT couldn't be considered a proliferation test in absence of a treatment: MTT or XTT assay  could be a better choice if you want to evaluate a compound cytotoxicity. 

Hi Abraham, you can label cells with CellTracker Green (IVGN). This is a non-toxic intracellular stain that is carried on by the progeny (see publication).

Titrate the labeled cells into 96-well plates, measure the fluorescence do a BCA protein assay on the cells or count them.

You will now have a standard curve of cell florescence vs cell number (or cell protein).

Now you can label your cells with the CellTracker, plate them and not have to trypsinize them because you know how many cells are there by fluorescence as compared to your standard curve. 

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Thanks for your answers guys. Would research your suggestions and consider each as an option.

And Carlo, I would be using the traditional Hemocytometer counting method. We don't have an automated counter at our lab, unfortunately :(

when i trypsinizing the cells in 96 well late the cells are not trypsinizing but they are clumped and aggregate .This makes diffficult to count .