I'm constructing an mRNA (cDNA) library and I'm losing the cDNA during column fractionation. Can I replace the column with an electrophoresis gel for size fractionation?
I'm making my research on metagenomics and I want to find the source of the command 'vsearch merge-pairs' that used to be 'vsearch join-pairs'' How can I cite that?
28 June 2024 3,960 1 View
what are novel cryptographic protocols that enhance email security?
13 June 2024 7,090 2 View
I need to separate my drug from a sample with activated sludge and residual water
17 April 2024 9,601 2 View
Hey! I'm looking for ways to compare two kinetic curves over time. My data is composed of neutralizing antibody results during 11 points (vaccine kinetics) in each group (control and...
29 February 2024 9,979 3 View
On the page I currently cannot download it, or if you can guide me to generate it.
21 February 2024 4,374 0 View
I need to know if there are measurements or studies on the affinity of serotonin (endogenous) for the 5-HT1 and 5-HT2 receptors
03 February 2024 5,878 3 View
Can you please tell me how you induce insuline resistance. My protocol does not work. Seems like i'm missing something. i seed my cells at 1 million in petri culture and starved them for 24h. I...
16 January 2024 5,782 1 View
Good day everyone. I'm working with eDNA Metabarcoding for freshwater fishes in Brazil, I ran tests amplifying positive controls and samples with the primer MiFish-U (Miya et al 2015) alone (no...
11 January 2024 5,109 2 View
Hello, We designed a series of tests that aim to assess knowledge in first-year university students, and went through the process of validating each one of them (construct validity, through EFA,...
01 January 2024 4,251 4 View
Hello everyone, I'm currently working with chromatograms on a Shimadzu system and am seeking assistance in converting files saved in either .CDF or .qgd formats to .d format for use in Mass...
12 December 2023 5,663 2 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
I am new to Micromechanics and having similar problem with understanding the implementation of the formula's. I would appreciate if anyone can guide me on how to go about getting a scalar value...
30 July 2024 969 0 View
When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!
29 July 2024 488 0 View
Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...
23 July 2024 1,329 5 View
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,761 5 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
22 July 2024 155 4 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View
> library(meta) Error: package or namespace load failed for ‘meta’ in loadNamespace(i, c(lib.loc, .libPaths()), versionCheck = vI[[i]]): there is no package called ‘numDeriv’
18 July 2024 3,536 0 View
I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...
17 July 2024 6,213 4 View