I am trying to create cDNA library for yeast two hybrid system using invitrogen kit. It looks amazing at this moment but one problem is bothering me. It looks that I have 5’-UTR regions. Invitrogen recommends adding adaptors that helps translate in 3 different frames but it's not helping. 5UTR looks like a problem to me. Can anyone help me on this? I will appreciate any advises and suggestions.

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