We want to make a secreted protein in the membrane so that we can use this for cDNA immunization for nanobody generation.

We already tried using the recombinant material of that protein for immunization, but we are not successful, it might be due to the recombinant material is not in the correct conformation so now we're trying to do so using cDNA for correct confirmation of epitope.

Thanks a lot for your time and help.