In general, CD11b is considered a pan-macrophage marker, although this distinction is not earned. It is expressed on a huge variety of leukocytes and can be upregulated on activated cells irrespective of their naive expression status. CD11c is generally expressed by dendritic cells, but again, is highly activation-dependent, so be careful with these distinctions. The two markers do help to differentiate some subclasses of DCs, with myeloid-lineage DCs generally co-expressing both, while lymphoid-lineage DCs tend to lack CD11b.

they are major compatibility component (MHC) markers in human body cd means cluster of differentiation . they marks the immunity

http://en.wikipedia.org/wiki/List_of_human_clusters_of_differentiation

Thx a lot!!!

cd11c on B cells was found to be involved in autoimmune disease in women.

here is an interesting paper

http://bloodjournal.hematologylibrary.org/content/118/5/1305.abstract

Integrins are heterodimeric integral membrane proteins composed of an

alpha chain and a beta chain. CD11b is the integrin alpha M chain, CD11c the integrin alpha X chain. CD11b is important for the adherence of neutrophils and monocytes to stimulated

endothelium, it also plays a role in phagocytosis of complement coated particles.

HI of all, How about this two markers on murine dendritic cells surface

Although some will disagree, the general agreement right now is that DC will express both cd11b and cd11c. I don't wish to be rude, but what people have told you above regarding MHC is not true. These are completely different molecules.

CD11c, it should be noted, is absent on plasmacytoid DC; see http://bloodjournal.hematologylibrary.org/content/98/8/2574.full

Thanks

In general, CD11b is considered a pan-macrophage marker, although this distinction is not earned. It is expressed on a huge variety of leukocytes and can be upregulated on activated cells irrespective of their naive expression status. CD11c is generally expressed by dendritic cells, but again, is highly activation-dependent, so be careful with these distinctions. The two markers do help to differentiate some subclasses of DCs, with myeloid-lineage DCs generally co-expressing both, while lymphoid-lineage DCs tend to lack CD11b.

F4/80 is useful in conjunction with CD11b to avoid granulocytes, I should add; Christina makes a great point.

F4/80 is also expressed on some granulocytic cell populations. So be careful with it.

as regard to CD11b vs CD11c none of these single markers are informative. you have to combine it with others depending on your application

Chistina and Noel make the best points. CD11c and CD11b though are expressed on many kinds of cells, they are best used to distinguish various DC/ macrophage lineages of mice. A concise look on this is provided at http://rndsystems.com/DAM_public/6997.pdf

Thanks for pointing out the F4/80 marker as well! I often see it used almost interchangeably with CD11b, but it can provide some useful distinctions if you're trying to really identify or target a particular subset of cells. Just for general interest, it's also expressed on microglia in the brain (though to a lesser extent than CD11b).

Hello everyone, I want to study the state of maturation and activation of murine dendritic cells, is that I can freeze the lymph nodes or I should do it on freshly recovered lymph nodes of collection, if so under what conditions I must keep my samples, thank you again

please, How long put dendritic cells to migrate from the skin to the lymph nodes after skin infection, thank you

you should isolate the precursors of DC in marine bone marrow and cultivate them till maturation.

GMCSF and IL-4 for lots -o- DC.

theoretically yes but people usually prefer CD11b beads. It also depends on which macrophages you want to isolate under which conditions and for what aim

Hello, thank you for all the answers but may be I'm misspoken, I first want to treat my mouse and recovered lymph nodes and then study the dendritic cells maturation, my question is, can I freeze the lymph nodes and study the maturation of dendritic cells later or not I must do it just after the recovery of the lymph nodes, thank you agan

Saralou: you should NOT freeze the lymph nodes. While it will not cause new antigens on the cell surface (false-positive), it can cause LOSS of antigens from the cell surface (false-negative) and is not worth the risk. You also lose viable cells when you freeze and thaw, and lymphocytes are not very robust--they will die more easily than some other cell types. I found the best way to get cells was to take out the lymph nodes and gently scrub them through a 40 um nylon mesh cell strainer and rinse with PBS or media. You get a nice single-cell suspension.

Dr steel is right.

either you have to prepare single cell suspension immediately or alternatively you can put them on ice 4-8C for several hours(although outcome of the cells reduce and results will be somehow compromised) but don't freeze!!

CD11b is considered a pan-myeloid marker (expressed after granulocyte-monocyte progenitors (GMP) phase in the bone marrow). CD11c is a prefered marker for dendritic cells. But there is exceptions given the variety of tissue distribution of myeloid cells.

What is pan ?

Pan- as a prefix (Greek πᾶν, pan, "all", "of everything", "involving all members" of a group)

Thus, in scientific terminology, a pan-marker would be a marker for all types: e.g. a pan-neuronal marker is one that marks all neuronal cells regardless of subtype.

 If some a paper write down CD11b-CD11c+ cells expression in dendritic cells what does it mean?

S M Shamsul Islam,

In general, mouse DCs express CD11c but are negative for CD11b. Probably that's what they meant.