01 January 1970 0 256 Report

Anyone who has any case studies, in the medical, mental health or biology area, that they can send me for publication without our platform, will be welcome.

Permission to translate into Spanish or Portuguese.

[email protected]

More Rondineli Souza's questions See All
Is it safe to make a glycerol stock from Agrobacterium transformed cells from a 10-day plate?

Hello all. I transformed some Agrobacterium competent cells by electroporation and performed colony PCR to see the positive transformants. However, I performed the glycerol stock after 10 days of...

26 May 2024 9,304 3 View

Is it safe to purify plasmids of E.coli precultures from 2-month old plates?

Hello all. I purified some plasmids after doing precultures of strains from a 2-month old plate in -4 degrees. The PCR results were fine and I used theses plasmids to transform Agrobacterium...

26 April 2024 8,440 3 View

How has the efficiency of photovoltaic cells improved over time?

On the use of solar energy in homes.

07 April 2024 9,465 2 View

Can someone indicates me a database about meteorological variables per country?

I'm looking for variables like solar irradiation, wind speed, humidity, temperature and precipitation

06 April 2024 1,926 2 View

How to prepare dibenzo[a,h]anthracene stock solution? Which solvent to use?

I have trouble solubilizing the analytical standard of dibenzo[a,h]anthracene (a polycyclic aromatic hydrocarbon purchased from Sigma-Aldrich) to prepare a stock solution. I have already tried...

01 April 2024 5,768 3 View

Mitosox in mitochondrial extract?

Hi everyone, I was wondering if someone has a protocol for using MitoSOX in mitochondrial-enriched extracts. MitoSOX can migrate to the nucleus and generate an inaccurate response about...

24 March 2024 466 0 View

How can I filter kraft lignin?

I have prepared a liquid medium for fermentation with kraft lignin in water, but the lignin was not completely dissolved. After fermentation, I am wondering about the best way to filter out the...

04 January 2024 7,479 1 View

Which stain can be used for visualizing qunencher-tagged DNA oligonucleotide using gel electrophoresis?

I am trying to see a quencher (BHQ)tagged synthetic DNA oligonucleotide on an agarose gel...I used EtBr for the same...I was able to visualize the strands that I have (not tagged by BHQ), but I...

20 December 2023 2,128 0 View

What the best protocol for protein extraction from human neutrophils?

I've tried to use Ripa buffer containing 0.1% SDS + 10% of proteinase inhibitors combined with 3 cycles of freeze/thaw (Dry ice with isopropanol/Dry bath 70ºC), followed by 16.000rcf for 10...

17 December 2023 3,030 1 View

Does anybody know how to make homemade ABI 3500 buffer ?

Ineed a recipe.

13 December 2023 9,782 0 View

Similar questions and discussions
• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

I am looking for the permission to use the OLBI survey. Does anyone know where I can obtain that information?

I have reached out to the original creator Dr. Demerouti but have had no luck with a response. I am completing my IRB form and need the information or permission by tomorrow. I know short notice,...

29 July 2024 3,684 0 View

How gold procurement?

https://www.cleanteqwater.com/resources/case-studies/gold-mine-australia/

29 July 2024 5,161 1 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Do you know of any online international conferences that offer free discussions?

Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.

28 July 2024 6,501 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close