How to identify BL21 vs DH5a strain?

Is it possible to identify E.coli strains using PCR-based methods? How does the identification of E.coli strains work? Is it based on 16srRNA and phylogeny study or ...?

11 December 2018 5,990 4 View

The best protocol for antibody denaturation

Hi, we want to extract DNA from a solution that contains antibody. our results showed that our protocol can't lead to denature the antibody that well. We have searched a lot and find the protocols...

06 July 2017 5,206 4 View

Do Au nanoparticles have measurable electrochemical peak in HNO3?

I'm trying to find a suitable supporting electrolyte for simultaneous recording electrochemical behaviour of Au NPs and CdS QDs using DPV, LSV or SWV method.

09 October 2016 3,351 6 View

Is there a TRIzol-based protocol for microRNA isolation from serum samples without using any commercial kit or purification column?

How much serum would be sufficient for obtaining a good yield?

31 December 2015 6,739 6 View

Is there any other suitable buffer for EDC/NHS reaction beside MES buffer?

I need to use EDC/NHS coupling in my assay and I don't have MES buffer. Does anyone have used other buffers such as PBS? Is is efficient?

04 May 2015 6,706 8 View

Which kit you recommend for microRNA isolation from blood?

03 April 2015 7,282 7 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View