05 May 2017 1 6K Report

1) Chromosome Translocation: chromosome microarray + FISH? + CGH?

2) Determination of the levels of gene expression of Gene XYZ with or without hormone treatment in a cell line: FISH + (RT-PCR)?

3) Presence of a K-ras mutation in a set of head and neck tumors: Sanger  + NGS?

4) Gene Amplification: PCR + FISH? + qPCR. Is FISH appropriate here?

5) Identifying CD4 and CD8 positive T cells: Flow cytometry + immunohistochemistry

Please feel free to suggest any other techniques for any of the experiments above. I just want a list of all possible techniques for these specific assays.

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