How to export ZEN 3D time series?

I want to export 3D time series movie from ZEN. I found that I can only export one 3D image with its X or Y rotation instead of 3D-time series? I tried to use Fiji with the function of Z...

07 February 2021 5,365 1 View

HPLC separation for methyl salicylate, camphor, thymol and menthol?

I have been recently trying to develop a method to separate the above compounds. Most methods tried were adapted from literature using RP C8 or C18 column with ACN/MeOH and buffered water at pH 2...

28 December 2020 4,426 6 View

How to extract the different components at a specific frequency between two spectra?

Dear everyone: Hello! Sorry that my question may take up your valuable time. Recently I have a technical problem in the data processing. We have conducted a series of wind tunnel tests in order...

18 December 2020 8,847 2 View

Has anyone extracted plasmid DNA from the Dmp1-GFPtopaz plasmid?

I just bought a plasmid from addgene, the name is DMP1-GFPtopaz. However, I could not extract the vector DNA by using Qiagen DNA purification kits from the bacteria. the bacteria growth was very...

05 November 2020 2,458 3 View

Machine Learning and Big Data Analytics Tools Question?

What would be the best big data analytical tools to determine changes in behavioral health (e.g., addiction behaviors)? The data used to predict behavioral health changes are secondary data which...

30 September 2020 9,522 8 View

May I know is Triple Negative cell line MDA-MB-468 is auto fluorescence ?

I am doing immunofluorescence using MDA-MB-468 cell to determine ICAM-1 and E-cadherin with Santa Cruz Biotechnology ICAM-1 (G-5) mouse anti-human (Primary antibody), Santa Cruz Biotechnology...

27 August 2020 1,641 4 View

What is a good rice variety to research on red, brown, black and white rice towards antioxidant properties and anti-alphaglucosidase on diabetes?

We will be conducting research on colored rice--red, brown, black and white rice and utilize FRAP, superoxide assay and hydroxyl scavenging assay to determine antioxidant activity and correlate it...

20 April 2020 7,334 3 View

Can we use pig imune serum to perform a passive immunization in mice?

We have vaccinated pig with antigens and then, we would like to know whether we can use mice to perform a passive protection assay by using the pig immune sera. Do you know pig macrophages can...

15 April 2020 2,510 2 View

How to statistically compare two different measurements for the same factor in a logit regression?

My key independent variable in my regression (logit) is the product's degree of spanning. I have two different measurement methods for this factor. And how can I statistically compare?

10 April 2020 3,679 3 View

Where is freestream velocity used in the noise prediction of helicopter rotor in forward flight condition with an observer fixed relative to rotor?

I am not so clear the use of freestream velocity in FWH equation when helicopter rotor was in forward flight condition and observer position was fixed relative to the rotor. The description of...

21 March 2020 8,631 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Agarose gel purification pre MinION sequencing?

Dear all, we performed an agarose gel purification with MinElute kit (QIAGEN) to extract bands between 3500 bp and 6000 bp for further sequencing with MinION. After the purification process,...

16 February 2021 5,178 1 View

Trouble shooting with his tagged protein expression in E.Coli?

i have issues in expressing a chicken protein in BL21 cells with his tag. I do not see induction even at 18 degrees. Any suggestions

15 February 2021 8,939 3 View

Why samples do not run in resolving gel?

Hello everybody, I have a problem with SDS-PAGE and I do not know why my samples stop at the top of the resolving gel and they do not run through it. The percentage of gel is 15% and the size of...

14 February 2021 9,116 6 View

Low DNA yields with midiprep and maxiprep plasmid extraction kits but excellent results with miniprep kits. What can be a possible reason?

I need to extract large amounts of plasmid DNA for transfection into mammalian cell lines with as little as possible enterotoxins and I usually use the Qiagen Endofree plasmid maxi kit for the...

09 February 2021 3,779 6 View