I had initially used a hybrid Qiagen RNeasy miniprep/Trizol method to isolate my RNA, however due to the small amount of tissue (mouse skin separated into epidermal and dermal layers using 1M NaCl), it has yielded a small amount of RNA albeit pure.
As a result, I am seeking to re-precipitate the RNA eluted using the Qiagen miniprep kit with potassium acetate. However, will the quality of the RNA be okay to use in Q-PCR?
Yes.
I use sodium acetate, but the principle is the same.
After precipitation, wash the pellet gently with ice-cold 75% ethanol (add 300ul gently down the side of the tube, spin for 5-10mins, repeat). This removes most of the salts.
Redissolve pellet in appropriate volume of nuclease free water.
NOTE: reprecipitation usually loses some RNA, so factor that in to your calculations as to what volume to redissolve your pellet in.
I think once you have isolated an RNA the method of its isolation won't affect its use
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