Can you tell me a protocol to make transformed lyophilized E coli?

Martin Williams @Martin-Williams-6

10 November 2015 1 5K Report

I need to make Master E coli cultures but i cant keep them at -80. Is there any way to lyophilize transformed E coli?

Thanks

Pierre Béguin

Hello Martin,

Yes, recombinant E. coli strains can be lyophilized in the presence of calf serum in vacuum-sealed ampullae

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