Can you suggest some plant transformation vectors that I can use for the expression of GFP/YFP-tagged protein of my interest?

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H3K9Me2/3 western blots in S.pombe?

I have recently started working with heterochromatin . I am trying to quantify the H3K9Me levels but i am failing to do so. Sometimes i see faint duplex band . I do TCA Lysis and then follow up...

22 May 2024 2,071 2 View

What is the difference between sequence identity and percent identity?

Hello, I have come across a paper where it is stated that Sequence identity between these two proteins is ~36%. I performed BLAST and in the summary section, it is stated that that percent...

16 January 2024 1,478 2 View

Dock apoprotein with cofator Mg?

Hello, I am working on a protein that belongs to NUDIX family hydrolases and therefore requires Mg ion as a cofactor. But the protein that I have downloaded from PDB is an apoprotein. I want to...

11 January 2024 8,105 2 View

uess. Please give a comment on this for the study of complex with water?

iti

03 January 2024 2,019 3 View

What is the basis of a straight line fitting of Kamlet-Taft plot (calculated emission vs observed emission plot) ?

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03 January 2024 6,617 1 View

How to generate decoys for validation of generated pharmacophore hypothesis?

Dear all, I have developed a pharmacophore hypothesis using receptor cavity since my protein has no co-crystallized ligand. I , now, aim to validate it using a set of actives and decoys. I tried...

30 November 2023 1,424 0 View

The files generated from DUD-E are in '.picked' format. How can I use them in Schrodinger for hypothesis validation?

Also, is there a way to convert SMILES into IUPAC or common name of the compound?

30 November 2023 304 2 View

I need an urgent help for ctm4xas simulation?

I am downloading the program properly but after giving the significant command the program is not executing. It is showing "Error while evaluating Menu callback". I am totally blank about...

29 October 2023 4,917 2 View

Can I dock a protein and a diadenosine polyphosphate nucleotide(protein's ligand)? If yes, then how?

I have a protein that binds to ATP and another nucleotide. I want to dock them so that I can find out the binding site residues in the protein. Which tool can I use?

10 October 2023 8,217 4 View

Please share how to draw an inverse spinel oxide crystal structure, like CoFe2O4?

I have tried through Vesta but the files not producing proper structUre in the octahederal positions

03 October 2023 1,985 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

How to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field in Techplot?

Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You

02 August 2024 3,615 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?

31 July 2024 7,921 5 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to back transform the results generated from analyses using log transformed with In(X+1) data?

I am conducting my analysis using SPSS. I log transformed my data using In(X+1) as my data contain zero values. However, when I want to back transform the regression coefficients generated from my...

31 July 2024 7,860 3 View