Can you suggest some plant transformation vectors that I can use for the expression of GFP/YFP-tagged protein of my interest?

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Precipitation of Pcl from polymeric solution ?

We working on PCL nano particles 1).Ketoprofen (10mg)+ dmso(5ml) Pcl (10mg)+ DCM(5ml) 2).Water 10 ml+ 10 mg When we mix both 1+2 after solublization (400 rpm) Why precipitation of polymer occurs?

09 December 2020 5,033 11 View

Can anyone suggest me an industry working in Biostimulants ?

it would be very helpful if anyone would suggest industries working in biostimulants.

25 October 2020 5,824 2 View

Queries regarding Antibody labelling ?

Hello Everyone. I was trying to label antibody with Cat# MO-L007 - Cystein reactive monolith series antibody labelling kit. I followed every steps that the protocol states. But in the final step...

26 September 2020 3,439 1 View

I am getting very high value for permeability through membranes?

i am working on gas separation based on flat sheet membranes. i was trying to measure the n2 permeability through the membranes and the value i am getting is very high. and the flow rate is also...

12 September 2020 8,632 11 View

How to calculate the permeability in barrer units??

HI, i am working gas separation using flat sheet membranes and I am new to this topic. I tried to find the permeability of nitrogen in the membrane by varying the inlet pressure and measuring the...

06 September 2020 4,656 3 View

HOw to conduct Battery sizing for Wind Farms and PV Farms?

Considering a battery for power output smoothing, how is it possible to calculate the capacity of a battery that can be used in a wind farm and a pv farm?

19 July 2020 5,213 1 View

I am interested in locating nutrients essential for photosynthesis. How can I see the chloroplast of lemongrass leaf using SEM ?

I am interested in locating nutrients important for photosynthesis. How can I locate them in lemongrass leaf using SEM or some other protocol.

07 July 2020 6,779 3 View

I am interested in locating nutrients essential for photosynthesis. How can I see the chloroplast of lemongrass leaf using SEM ?

I am interested in locating nutrients important for photosynthesis. How can I locate them in lemongrass leaf using SEM or some other protocol.

07 July 2020 2,194 3 View

How do we calculate mechanical stress using VASP Calculations for Ab Initio Molecular Dynamics?

If we want to uniaxially strain a structure incrementally, and calculate the corresponding uniaxial stress at every strain point, how should we use the VASP output files for this purpose? I have...

04 April 2020 9,747 4 View

How do we extract data from netcdf file that overlaps with shapely Polygon geometry in python?

I have data for precipitation in netcdf file format and a shapely polygon geometry. I want to extract only the data that overlaps with the polygon. Any idea how we do that in python?

01 March 2020 9,938 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View