How to conduct analysis for experiment data with a mediation model and time series data?

Hi everyone! I am struggling with experiment data analysis as I am not from psychology background. My key research questions are 1. The impact of leader possible self (LPS) (X1) on...

05 July 2020 6,348 3 View

What is the intracellular distribution of Matrix Metalloproteinase (MMP)-9 in fast motor neurons?

What is the intracellular distribution of Matrix Metalloproteinase (MMP)-9 in fast motor neurons (if possible, which organelle)? Do they found in the axon terminal? Additionally, do microglia...

28 September 2015 7,248 3 View

What is the prefered/best guage of Hamilton needle used for mice brain injections?

I am recently planning to do some stereotaxic injections to some mice brain nuclei. Due to the various choices (in particular the G number) of Hamilton needles, I was wondering which one would be...

28 February 2015 5,854 3 View

What is the extraction protocol for chlorophyll with high purity?

I am currently working with flowering plants, as I need to quantify the content of chlorophyll (particular subtype, e.g. chlorophyll a) in a specific leaf after its extraction, I was wondering...

12 December 2014 2,210 12 View

Any suggestions to get the characteristic red pigment expressed by Microsporum gallinae?

Microsporum gallinae ATCC 90749; received strain from ATCC and have grown on Sabouraud dextrose agar at various temperatures.

03 April 2014 249 2 View

Can anyone help with electrophoresis gel reuse?

After the first (agarose or SDS-PAG) electrophoresis, if I run the same gel again with an-overtime so that everything in the gel will now in theory come off the gel (i.e. electro-rinsing), then...

05 March 2014 886 10 View

Anyone have experience with immunoblot for watercrystal damaged samples?

If watercrystal damage happened in my whole brain samples, would there be any significant differences in the result of my immunoblotting between step A and step B (which are as follows)? Step A:...

11 February 2014 8,381 2 View

Is transitional analysis in large scale chromatography being used in industry?

looking for information.

01 January 1970 3,013 1 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View