Is there any reliable institute that analyse the extract for isolation and characterization of bioactives? I have found chloroform fraction (through kupchan protocol) of plant the most toxic one against seed germination and seedling growth in four weeds in wheat crop. Now I want to point out the toxic compounds in that fraction. What are different possibilities for me for analysis of my sample?
First you can do fractionation of the extract using column chromatography on silica gel and after that to analyze the fractions by GC-MS or UHPLC-MS.
The components are very possibly terpenes components of essential oils.
Hai Huma, In the chloroform fraction you got from the plant may contains so many compounds. If want to characterize the compound which is responsible for your activity
you have to do chromatographic techniques. Without any sophisticated instruments you can simply do TLC, and Column chromatography. It will definitely fractionated into more purified compounds and there after you can give the sample for GCMS analysis.If it is a known compound you can compare with the mass spectra with those of reported compounds.If its feel to be novel you have to go for 1H,13C NMR.I dont know about any paid service..even if it is there they will only do analysis and not purification
Yes lots of them are there - I mention one is at New Delhi, India - it would help if you could mention a preffered location
Advanced Instrumentation Research Facility (AIRF) -JNU (http://www.jnu.ac.in/AIRF/)
Yes, many organizations/Institutes give facilities to analyse the isolated compounds on request undersigned by the concern authority. You can visit such organization.
You can do this work easily at HEJ Karachi, PCSIR Lahore or MBC,PCSIR Peshawar Labs.