Inhalation of synthetic vaporizer is harmful to children. I want to detect the injured vial organs caused by inhalation of transfluthrin vapor in mice by in vivo imaging.
Perhaps you could take advantage of the enhanced permeability and retention (EPR) effect. Inflammation causes the vasculature to have increased permeability to nanoscale particles, so that they accumulate in the site of inflammation. You could use 100-nm fluorescent nanoparticles, which are available commercially.
Thank you sir for your advice Adam B Shapiro
Sir, will you please suggest me the commercial brand for 100-nm fluoroscent nanoparticle with literature relating to your suggestion?
There are numerous commercial sources. Here are a few.
https://www.perkinelmer.com/category/fluorescent-nanoparticles
https://www.lab261.com/pages/fluorescent-polystyrene-microspheres?gclid=Cj0KCQjwla7nBRDxARIsADll0kBXkKXd2QssFo09l9WWV_0Hlq0Sq5IVGMA6l8N6DcwewSJauR69wDcaAovPEALw_wcB
https://www.sigmaaldrich.com/life-science/biochemicals/biochemical-products.html?TablePage=15933803
https://www.cd-bioparticles.com/product/fluorescent-gold-nanoparticles-list-177.html?gclid=Cj0KCQjwla7nBRDxARIsADll0kCMl1PGIsNP13fuwfJbhP8Q_wnc1R4Wio-9akm4Dr9mcejwy1moa7saApEtEALw_wcB
Inflammation generally links to NFkB/IkB/Ikks signaling. A key experiment is to evaluate time/exposure scale and detect p65/Ikbs and Ikks in presence of H3P in your vivo targets. To explore, use signal, time, freezing, cryosection conditioning. You can check also for total animal imaging. Mice might not be the best candidates. Something smaller….or a cell model…