Can you perform multiplex PCR using biorad t100 thermal cycler?

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Can Document Analysis be used as the only method of research?

I have read that it is better to use other research methods alongside document analysis for triangulation, although it can also be used as a stand-alone method.

18 July 2024 4,218 0 View

In kalman filter, it is logical to have the observation variance equale 0 ?

I am working on implementing a Kalman filter integrated with ARMA parameters, as described in the article "Predicting Time Series Using an Automatic New Algorithm of the Kalman Filter"...

12 July 2024 8,116 2 View

Hi i m workiking with hct116 resistant and naïve cells after passage half of my cells die can anybody help?

thanks

25 June 2024 4,667 0 View

How to calculate the activation energy for grain and grain boundaries?

how to calculate the activation energy for grain and grain boundaries?

18 May 2024 5,413 0 View

On average, how many rounds of revision does a manuscript undergo before being accepted in an SSCI journal?

This research question seeks to determine the average number of revision rounds a manuscript typically undergoes before being accepted by an SSCI journal. Understanding this average can help...

18 May 2024 5,815 6 View

How to handling asymmetric data sets when one group has significantly more responses than the other?

(1) How to analyze relation between two variables in which data is obtained on the ordinal scale from two different groups and the data sets are asymmetric, where one group has significantly more...

15 May 2024 4,950 5 View

Hi! Please suggest the appropriate tests for hypothesis testing?

My Phd is in HR area. I want to study the impact of independent variable on dependent variable. And study whether there is significant positive relationship between IV & DV. in such...

24 March 2024 1,932 4 View

Monitoring of microalgae growth with OD?

Hi, Could anyone tell me how to do to calculate optical density at 680 nm of microalgae culture when the suspension is diluted several times? Exemple: OD at 680 for a strain, in day 4 was 1.564...

20 March 2024 1,043 0 View

What are the limitations of Dynamic Bayesian Network (DBN)?

Hello everyone I am looking for the limitations on applying dynamic Bayesian networks (DBN); please provide scientific papers in this context.

19 February 2024 6,009 0 View

What is the best link/web for learning python programming language for conducting exploratory analysis quickly?

Hello everyone! I want to learn python programming language for conducting exploratory analysis of the Skytrax reviews about airlines. Can anyone guide me which website or platform is the best for...

29 January 2024 4,455 5 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View