I am looking to know if it is suitable to package 3rd generation viral plasmids (pGIII) with a 4th generation packaging system i.e GIPz/TRIPz. Any 'rules' to follow would be more than helpful with regards to viral production in such a way.
I'm analyzing abundances from various ecological surveys. So data was collected across multiple independent years from three sites within each year. The years fall under two temperature...
26 June 2024 2,798 2 View
I am working with ecological count data where the raw data is divided into multiple sites among sampled years. So you have site 1,2,3...etc for year 1999, 2000, 2001, etc. Each year is within...
28 April 2024 8,675 0 View
I am doing a study focusing on analyzing differences in fish assemblages due to temperature extremes. I calculated Shannon diversity, evenness, richness, and total abundance for each year sampled....
03 March 2024 4,660 1 View
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19 February 2024 5,609 0 View
I apologize in advance as I am new to community data analysis. I am currently analyzing differences in fish assemblages due to temperature extremes. I am looking at data over multiple years(the...
26 September 2023 1,398 3 View
Hello, I have a question if I may. I am looking to run a statistical test to see the differance in mood states (measured using POMS then working out TMD) at 3 time points. I have one independent...
07 May 2023 6,024 3 View
Sano, K., & Shimada, H. (2021). Goal orientation and achievement goals in self-regulated learning: a latent profile analysis. Educational Psychology, 41(5), 843-863....
06 April 2023 9,707 0 View
Hi everyone, i have a abaqus model where in step one a interference fit is solved and in step 2 i want to add a displacement to the model. For some reason I end with the Warnings: The system...
02 September 2021 6,360 2 View
Hi, just a small query. In M+ when running logistic regression, you define nominal covariates using the 'define' command, for example, were a (gender) var is coded 0 = male/1 = female...
07 February 2020 1,989 2 View
I am currently doing a service improvement project at university, based on improvement of fluid balance charts, regarding monitoring and recording, more specifically for surgical high care...
20 September 2019 744 3 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,573 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,648 1 View
I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...
30 July 2024 1,040 1 View